![]() For IEF, proteins will move either towards the cathode or anode depending on their pI.SDS-PAGE gives ALL proteins a net negative charge, so they will always travel towards the anode.For native PAGE, an alkaline buffer is usually used, which gives most proteins a negative charge.Remember, these are all electrolytic cells, so the cathode is negative and the anode is positive.What does this mean for IEF, NATIVE-PAGE and SDS-PAGE? This is why you see bubbles flowing from the liquid, as H2O is lysed into H2 and O2. ALL ELECTROPHORETIC METHODS ARE RUN ON ELECTROLYTIC CELLS.The cathode is now connected to an external negative pole.The anode is now connected to an external positive pole. ![]() We need to provide an external voltage source to drive this nonspontaneous reaction. The cathode (source of reduction) is positively charged.These drive SPONTANEOUS reactions, where Ecell > 0 (and deltaG The cathode will ALWAYS attract cations as electrons pile up, and the anode will ALWAYS attract anions as newly generated cations pile up.Electrons ALWAYS flow from anode to cathode.Oxidation ALWAYS happens at the anode, reduction ALWAYS happens at the cathode, no matter what.Here's my attempt at encompassing it all: /r/GAMSAT - Australian & UK Medical school Admission Test.P/S = Psychological, Social, and Biological Foundations of Behavior (Tentative) AAMC Sample FL Score ConversionĬ/P = Chemical and Physical Foundations of Biological SystemsĬARS = Critical Analysis and Reasoning Skillsī/B = Biological and Biochemical Foundations of Living Systems # Correct -> Scaled Score Converter for AAMC Material These posts will be removed and the user banned without warning, subject to the discretion of the mod team Learn MoreĪll of the above rules are subject to moderator discretion High Yield MCAT Links You have signed an examinee agreement, and it will be enforced on this subreddit.ĭo not intentionally advertise paid or free products or services of any sort. We have one "stickied" post for each exam and score release day, contain all test day discussion/reactions to that thread only.ĭo not discuss any specific information from your actual MCAT exam. For an example format for submitting pictures of questions from practice material click hereĭo not link to content that infringes on copyright laws (MCAT torrents, third party resources, etc).ĭo not post repeat "GOOD LUCK", "TEST SCORE", or test reaction posts. These are considered spoilers and should be marked as such. ![]() Be nice to each other, hating on other users won't help you get extra points on the MCAT, so why do it?ĭo not post any question information from any resource in the title of your post. Rudeness or trolling will not be tolerated. Please message the moderators with your skills/ideas! MCAT RESOURCES & INFO Study Groups Want to help us improve this subreddit or tell us about a new resource we can add to the sidebar? Below you will find our forum rules, resources, and more. ![]() We request that you read the sidebar COMPLETELY before you post. r/MCAT is a place for support, discussion, advice, social networking, news, study tips and more. The MCAT (Medical College Admission Test) is offered by the AAMC and is a required exam for admission to medical schools in the USA and Canada. Welcome to the BEST place for MCAT prep and practice materials.
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